Pre-marked chromatin and transcription factor co-binding shape the pioneering activity of Foxa2.

ABSTRACT: Pioneer transcription factors (PTF) can recognize their binding sites on nucleosomal DNA and trigger chromatin opening for recruitment of other non-pioneer transcription factors. However, critical properties of PTFs are still poorly understood, such as how these transcription factors selectively recognize cell type-specific binding sites and under which conditions they can initiate chromatin remodelling. Here we show that early endoderm binding sites of the paradigm PTF Foxa2 are epigenetically primed by low levels of active chromatin modifications in embryonic stem cells (ESC). Priming of these binding sites is supported by preferential recruitment of Foxa2 to endoderm binding sites compared to lineage-inappropriate binding sites, when ectopically expressed in ESCs. We further show that b

SUBMITTER: Cernilogar FM 

PROVIDER: S-EPMC6753583 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature