The HMGA2-IMP2 Pathway Promotes Granulosa Cell Proliferation in Polycystic Ovary Syndrome.

ABSTRACT:

Context

The high mobility group AT hook 2 (HMGA2) gene was previously identified in a genome-wide association study as a candidate risk gene that might be related to polycystic ovary syndrome (PCOS). Whether HMGA2 contributes to promoting granulosa cell (GC) proliferation in PCOS remains unknown.

Objective

We sought to determine whether HMGA2 is involved in the ovarian dysfunction of PCOS and in the mechanism of increased GC proliferation.

Patients and cells

mRNA expression was analyzed in ovarian GCs from 96 women with PCOS and 58 healthy controls. Immortalized human GCs (KGN and SVOG cells) were used for the mechanism study.

Main outcome measures

mRNA expression in ovarian GCs was measured using quantitative RT-PCR, and KGN cells were cultured for proliferati

SUBMITTER: Li M 

PROVIDER: S-EPMC6753588 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature