Effect of Ice Nucleation and Cryoprotectants during High Subzero-Preservation in Endothelialized Microchannels.

ABSTRACT: Cryopreservation is of significance in areas including tissue engineering, regenerative medicine, and organ transplantation. We investigated endothelial cell attachment and membrane integrity in a microvasculature model at high subzero temperatures in the presence of extracellular ice. The results show that in the presence of heterogeneous extracellular ice formation induced by ice nucleating bacteria, endothelial cells showed improved attachment at temperature minimums of -6 °C. However, as temperatures decreased below -6 °C, endothelial cells required additional cryoprotectants. The glucose analog, 3-O-methyl-D-glucose (3-OMG), rescued cell attachment optimally at 100 mM (cells/lane was 34, as compared to 36 for controls), while 2% and 5% polyethylene glycol (PEG) were equally eff

SUBMITTER: Tessier SN 

PROVIDER: S-EPMC6753837 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature