Urine proteome changes in rats subcutaneously inoculated with approximately ten tumor cells.

ABSTRACT:

Background

Biomarkers are changes associated with the disease. Urine is not subject to homeostatic control and therefore accumulates very early changes, making it an ideal biomarker source. Usually, we have performed urinary biomarker studies involving at least thousands of tumor cells. However, no tumor starts from a thousand tumor cells. We therefore examined urine proteome changes in rats subcutaneously inoculated with approximately ten tumor cells.

Methods

Here, we serially diluted Walker-256 carcinosarcoma cells to a concentration of 10²/mL and subcutaneously inoculated 0.1 mL of these cells into nine rats. The urine proteomes on days 0, 13 and 21 were analyzed by liquid chromatography coupled with tandem mass spectrometry.

Results

Hierarchical cluster

SUBMITTER: Wei J 

PROVIDER: S-EPMC6753921 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature