RNA-based qPCR as a tool to quantify and to characterize dual-species biofilms.

ABSTRACT: While considerable research has focused on studying individual-species, we now face the challenge of determining how interspecies interactions alter bacterial behaviours and pathogenesis. Pseudomonas aeruginosa and Staphylococcus aureus are often found to co-infect cystic-fibrosis patients. Curiously, their interaction is reported as competitive under laboratory conditions. Selecting appropriate methodologies is therefore critical to analyse multi-species communities. Herein, we demonstrated the major biases associated with qPCR quantification of bacterial populations and optimized a RNA-based qPCR able not only to quantify but also to characterize microbial interactions within dual-species biofilms composed by P. aeruginosa and S. aureus, as assessed by gene expression quantification. qPC

SUBMITTER: Magalhaes AP 

PROVIDER: S-EPMC6754382 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature