Identification and validation of potential reference gene for effective dsRNA knockdown analysis in Chilo partellus.

ABSTRACT: Chilo partellus is an invasive polyphagous pest that has not been effectively managed with chemical pesticides. To select potential dsRNAs for use in an alternate control strategy, it is crucial to identify and evaluate stable reference genes for knockdown expression studies. This study evaluates the expression stability of seven candidate reference genes in C. partellus larvae fed on crude bacterially-expressed dsRNAs and purified dsRNAs at different time intervals, as well as the developmental stages and sexes. The expression stabilities of the reference genes were evaluated with different software programmes, such as BestKeeper, NormFinder, deltaCt, geNorm, and RefFinder. The overall results rank ELF as the most stably expressed reference gene when larvae were fed with crude bacteria-in

SUBMITTER: Adeyinka OS 

PROVIDER: S-EPMC6754392 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature