Subtractive inhibition assay for the detection of Campylobacter jejuni in chicken samples using surface plasmon resonance.

ABSTRACT: In this work, a subtractive inhibition assay (SIA) based on surface plasmon resonance (SPR) for the rapid detection of Campylobacter jejuni was developed. For this, rabbit polyclonal antibody with specificity to C. jejuni was first mixed with C. jejuni cells and unbound antibody was subsequently separated using a sequential process of centrifugation and then detected using an immobilized goat anti-rabbit IgG polyclonal antibody on the SPR sensor chip. This SIA-SPR method showed excellent sensitivity for C. jejuni with a limit of detection (LOD) of 131 ± 4 CFU mL^-1 and a 95% confidence interval from 122 to 140 CFU mL^-1. The method has also high specificity. The developed method showed low cross-reactivity to bacterial pathogens such as Salmonella enterica serovar Typhi

SUBMITTER: Masdor NA 

PROVIDER: S-EPMC6754509 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature