Structural basis for transcription activation by Crl through tethering of σ^S and RNA polymerase.

ABSTRACT: In bacteria, a primary σ-factor associates with the core RNA polymerase (RNAP) to control most transcription initiation, while alternative σ-factors are used to coordinate expression of additional regulons in response to environmental conditions. Many alternative σ-factors are negatively regulated by anti-σ-factors. In Escherichia coli, Salmonella enterica, and many other γ-proteobacteria, the transcription factor Crl positively regulates the alternative σ^S-regulon by promoting the association of σ^S with RNAP without interacting with promoter DNA. The molecular mechanism for Crl activity is unknown. Here, we determined a single-particle cryo-electron microscopy structure of Crl-σ^S-RNAP in an open promoter complex with a σ^S-regulon p

SUBMITTER: Cartagena AJ 

PROVIDER: S-EPMC6754549 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature