Genome-wide identification of Pseudomonas syringae genes required for fitness during colonization of the leaf surface and apoplast.

ABSTRACT: The foliar plant pathogen Pseudomonas syringae can establish large epiphytic populations on leaf surfaces before apoplastic colonization. However, the bacterial genes that contribute to these lifestyles have not been completely defined. The fitness contributions of 4,296 genes in P. syringae pv. syringae B728a were determined by genome-wide fitness profiling with a randomly barcoded transposon mutant library that was grown on the leaf surface and in the apoplast of the susceptible plant Phaseolus vulgaris Genes within the functional categories of amino acid and polysaccharide (including alginate) biosynthesis contributed most to fitness both on the leaf surface (epiphytic) and in the leaf interior (apoplast), while genes involved in type III secretion system and

SUBMITTER: Helmann TC 

PROVIDER: S-EPMC6754560 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature