Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens.

ABSTRACT: Queuosine (Q) is a complex tRNA modification widespread in eukaryotes and bacteria that contributes to the efficiency and accuracy of protein synthesis. Eukaryotes are not capable of Q synthesis and rely on salvage of the queuine base (q) as a Q precursor. While many bacteria are capable of Q de novo synthesis, salvage of the prokaryotic Q precursors preQ₀ and preQ₁ also occurs. With the exception of Escherichia coli YhhQ, shown to transport preQ₀ and preQ₁, the enzymes and transporters involved in Q salvage and recycling have not been well described. We discovered and characterized 2 Q salvage pathways present in many pathogenic and commensal bacteria. The first, found in the intracellular pathogen Chlamydia trachomatis, uses YhhQ

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC6754566 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature