Photosystem II oxygen-evolving complex photoassembly displays an inverse H/D solvent isotope effect under chloride-limiting conditions.

ABSTRACT: Photosystem II (PSII) performs the solar-driven oxidation of water used to fuel oxygenic photosynthesis. The active site of water oxidation is the oxygen-evolving complex (OEC), a Mn₄CaO₅ cluster. PSII requires degradation of key subunits and reassembly of the OEC as frequently as every 20 to 40 min. The metals for the OEC are assembled within the PSII protein environment via a series of binding events and photochemically induced oxidation events, but the full mechanism is unknown. A role of proton release in this mechanism is suggested here by the observation that the yield of in vitro OEC photoassembly is higher in deuterated water, D₂O, compared with H₂O when chloride is limiting. In kinetic studies, OEC photoassembly shows a significant lag p

SUBMITTER: Vinyard DJ 

PROVIDER: S-EPMC6754581 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature