CREB deletion increases resilience to stress and downregulates inflammatory gene expression in the hippocampus.

ABSTRACT: The transcription factor CREB (cyclic AMP response element (CRE)-binding protein) is implicated in the pathophysiology and treatment of depression. Structural and functional studies in both animals and humans suggest that abnormalities of the hippocampus may play a role in depression. CREB regulates thousands of genes, yet to date, only a handful that mediate depression or antidepressant response have been identified as relevant CREB targets. In order to comprehensively identify genes regulated by CREB in the hippocampus, we employed translating ribosome affinity purification (TRAP) to detect actively translating mRNAs in wild type and CREB-deficient mice. Using Creb^loxP/loxP; Rosa^LSL-GFP-L10a mice, we conducted whole genome sequencing to identify transcripts only in

SUBMITTER: Manners MT 

PROVIDER: S-EPMC6754757 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature