Meg3-DMR, not the Meg3 gene, regulates imprinting of the Dlk1-Dio3 locus.

ABSTRACT: The imprinted delta like 1 homolog (DLK1) - thyroxine deiodinase type III (DIO3) locus regulates development and growth. Its imprinting regulation involves two differentially methylated regions (DMRs), intergenic-DMR (IG-DMR) and maternally expressed gene 3-DMR (Meg3-DMR). In mice, a maternal deletion of the IG-DMR leads to LOI in the locus, proving that the IG-DMR is a cis-acting imprinting control region of the locus. However, the Meg3-DMR overlaps with the promoter, exon 1 and intron 1 of the Meg3 gene. Because deletion of the Meg3-DMR inactivates the Meg3 gene, their roles in imprinting regulation of Meg3-DMR mice is unknown. Therefore, we generated two mouse models: Meg3^Δ(1-4) and Meg3^Δ(2-4), respectively targeting exons 1-4 and exons 2-4 of the Meg3 gene. A mate

SUBMITTER: Zhu W 

PROVIDER: S-EPMC6754764 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature