N^ω -(Carboxymethyl)arginine Is One of the Dominant Advanced Glycation End Products in Glycated Collagens and Mouse Tissues.

ABSTRACT: Advanced glycation end products (AGEs) accumulate in proteins during aging in humans. In particular, the AGE structure N^ω -(carboxymethyl)arginine (CMA) is produced by oxidation in glycated collagen, accounting for one of the major proteins detected in biological samples. In this study, we investigated the mechanism by which CMA is generated in collagen and detected CMA in collagen-rich tissues. When various protein samples were incubated with glucose, the CMA content, detected using a monoclonal antibody, increased in a time-dependent manner only in glycated collagen, whereas the formation of N^ε -(carboxymethyl)lysine (CML), a major antigenic AGE, was detected in all glycated proteins. Dominant CMA formation in glycated collagen was also observed by ele

SUBMITTER: Kinoshita S 

PROVIDER: S-EPMC6754957 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature