ERF1 mediates codon usage effects on mRNA translation efficiency through premature termination at rare codons.

ABSTRACT: Codon usage bias is a universal feature of eukaryotic and prokaryotic genomes and plays an important role in regulating gene expression levels. A major role of codon usage is thought to regulate protein expression levels by affecting mRNA translation efficiency, but the underlying mechanism is unclear. By analyzing ribosome profiling results, here we showed that codon usage regulates translation elongation rate and that rare codons are decoded more slowly than common codons in all codon families in Neurospora. Rare codons resulted in ribosome stalling in manners both dependent and independent of protein sequence context and caused premature translation termination. This mechanism was shown to be conserved in Drosophila cells. In both Neurospora and Drosophila cells, codon usage plays an im

SUBMITTER: Yang Q 

PROVIDER: S-EPMC6755126 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature