Combining Chemical Synthesis and Enzymatic Methylation to Access Short RNAs with Various 5' Caps.

ABSTRACT: Eukaryotic RNAs are heavily processed, including co- and post-transcriptional formation of various 5' caps. In small nuclear RNAs (snRNAs) or small nucleolar RNAs (snoRNAs), the canonical ^7m G cap is hypermethylated at the N² -position, whereas in higher eukaryotes and viruses 2'-O-methylation of the first transcribed nucleotide yields the cap1 structure. The function and potential dynamics of several RNA cap modifications have not been fully elucidated, which necessitates preparative access to these caps. However, the introduction of these modifications during chemical solid-phase synthesis is challenging and enzymatic production of defined short and uniform RNAs also faces difficulties. In this work, the chemical synthesis of RNA is combined with site-specific enzym

SUBMITTER: Muthmann N 

PROVIDER: S-EPMC6755138 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature