Evaluation of IC₅₀ levels immediately after treatment with anticancer reagents using a real-time cell monitoring device.

ABSTRACT: A real-time cell-monitoring analysis (RTCA) system was previously developed based on the change in impedance when cells attach and spread in a culture dish coated with a gold microelectrode array. However, the potential applications of this system have not yet been fully demonstrated. The purpose of this study was to test the utility of the RTCA system to determine the cytotoxicity of four anticancer agents in carcinoma cells. The results were compared with those of the conventional WST-8 assay at the endpoint to determine the potential of the RTCA system as a new real-time assay method to evaluate cytotoxicity. iCELLigence was used as the RTCA system in this study. Suspensions of oral squamous cell carcinoma (OSCC) cell lines were seeded (2×10⁴ cells/well) onto the E-plate (the

SUBMITTER: Hazekawa M 

PROVIDER: S-EPMC6755379 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature