Seroprevalence and molecular characterization of Mycobacterium bovis infection in camels (Camelus dromedarius) in the Delta region, Egypt.

ABSTRACT:

Aim

This study aimed to determine the prevalence rates of Mycobacterium infection in camel sera collected before slaughter and gross lesion tissue collected postmortem (PM) using enzyme-linked immunosorbent assay (ELISA), bacteriological culture, and polymerase chain reaction (PCR). In addition, serum samples from humans who had occupational contact with camels were tested by ELISA and sputum sample by culture.

Materials and methods

ELISA was performed on serum samples antemortem. In addition, bacteriological culture and PCR were conducted after PM. Tuberculosis infection was identified in humans who had contact with camels using ELISA for serum samples and culture for sputum samples.

Results

Tuberculous lesions were detected in 184 of 10,903 camels (1.7%). The

SUBMITTER: Elnaker YF 

PROVIDER: S-EPMC6755397 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature