Hypotonic Stress Induces Fast, Reversible Degradation of the Vimentin Cytoskeleton via Intracellular Calcium Release.

ABSTRACT: The dynamic response of the cell to osmotic changes is critical to its physiology and is widely exploited for cell manipulation. Here, using three-dimensional stochastic optical reconstruction microscopy (3D-STORM), a super-resolution technique, the hypotonic stress-induced ultrastructural changes of the cytoskeleton of a common fibroblast cell type are examined. Unexpectedly, these efforts lead to the discovery of a fast, yet reversible dissolution of the vimentin intermediate filament system that precedes ultrastructural changes of the supposedly more dynamic actin and tubulin cytoskeletal systems as well as changes in cell morphology. In combination with calcium imaging and biochemical analysis, it is shown that the vimentin-specific fast cytoskeletal degradation under hypotonic stress

SUBMITTER: Pan L 

PROVIDER: S-EPMC6755523 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature