Effects of challenge dose and inoculation route of the virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid-gestation.

ABSTRACT: Parameters such as pathogen dose and inoculation route are paramount in animal models when studying disease pathogenesis. Here, clinical findings, including foetal mortality, parasite transmission rates and lesion severity, and immune responses were evaluated in Asturiana pregnant heifers at day 110 of gestation challenged with a virulent (Nc-Spain7) Neospora caninum isolate. Four different doses of parasite tachyzoites were inoculated intravenously (IV1, 10⁷ parasites, n = 6; IV2, 10⁵, n = 6; IV3, 10³, n = 6; and IV4, 10², n = 5), and the subcutaneous (SC) inoculation route was also assessed for the dose of 10⁵ tachyzoites (SC, n = 6). In addition, a control group (n = 4 pregnant heifers) was evaluated. Foetal death was observed in al

SUBMITTER: Vazquez P 

PROVIDER: S-EPMC6755697 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature