Characterization of human norovirus binding to gut-associated bacterial ligands.

ABSTRACT:

Objective

Research suggests human norovirus binding to histo-blood group antigen (HBGA)-like molecules on enteric bacteria may enhance viral pathogenesis; however, the properties of these bacterial ligands are not well known. Previous work identified, but did not characterize, seven norovirus-binding bacteria. To further examine this bacteria-virus binding interaction, enteric bacteria were analyzed via Western blot with anti-HBGA antibodies and lectins targeting HBGA-associated sugar components. Virus overlay assays using capsids from six different human norovirus strains further identified responsible ligands and strain dependent binding properties.

Results

Each bacterial species possessed varying degrees of HBGA-like activity, and lectin binding further elucidated potenti

SUBMITTER: Almand EA 

PROVIDER: S-EPMC6755701 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature