Polar Effects of Transposon Insertion into a Minimal Bacterial Genome.

ABSTRACT: Global transposon mutagenesis is a valuable tool for identifying genes required for cell viability. Here we present a global analysis of the orientation of viable Tn5-Puro^r (Tn5-puromycin resistance) insertions into the near-minimal bacterial genome of JCVI-syn2.0. Sixteen of the 478 protein-coding genes show a noticeable asymmetry in the orientation of disrupting insertions of Tn5-Puro^r Ten of these are located in operons, upstream of essential or quasi-essential genes. Inserts transcribed in the same direction as the downstream gene are favored, permitting read-through transcription of the essential or quasi-essential gene. Some of these genes were classified as quasi-essential solely because of polar effects on the expression of downstream gene

SUBMITTER: Hutchison CA 

PROVIDER: S-EPMC6755753 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature