A strategy for the selection of monovalent antibodies that span protein dimer interfaces.

ABSTRACT: Ligand-induced dimerization is the predominant mechanism through which secreted proteins activate cell surface receptors to transmit essential biological signals. Cytokines are a large class of soluble proteins that dimerize transmembrane receptors into precise signaling topologies, but there is a need for alternative, engineerable ligand scaffolds that specifically recognize and stabilize these protein interactions. Recombinant antibodies can potentially serve as robust and versatile platforms for cytokine complex stabilization, and their specificity allows for tunable modulation of dimerization equilibrium. Here, we devised an evolutionary strategy to isolate monovalent antibody fragments that bridge together two different receptor subunits in a cytokine-receptor complex, precisely as th

SUBMITTER: Spangler JB 

PROVIDER: S-EPMC6755802 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature