α-Defensin HD5 Stabilizes Human Papillomavirus 16 Capsid/Core Interactions.

ABSTRACT:

Background

Human papillomavirus (HPV) is linked to nearly all cases of cervical cancer. Despite available vaccines, a deeper understanding of the immune response to HPV is needed. Human α-defensin 5 (HD5), an innate immune effector peptide, blocks infection of multiple sero-types of HPV, including high-risk HPV16. While a common mechanism of α-defensin anti-viral activity against nonenveloped viruses such as HPV has emerged, there is limited understanding of how α-defensins bind to viral capsids to block infection.

Methods

We have used cryo-electron microscopy (cryoEM), mass spectrometry (MS) crosslinking and differential lysine modification studies, and molecular dynamics (MD) simulations to probe the interaction of HPV16 pseudovirions (PsVs) with HD5.

Results

SUBMITTER: Gulati NM 

PROVIDER: S-EPMC6755940 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature