Dynamic m⁶A mRNA methylation reveals the role of METTL3-m⁶A-CDCP1 signaling axis in chemical carcinogenesis.

ABSTRACT: N6-methyladenosine (m⁶A) is the most abundant internal modification in mammalian mRNAs. Despite its functional importance in various physiological events, the role of m⁶A in chemical carcinogenesis remains largely unknown. Here we profiled the dynamic m⁶A mRNA modification during cellular transformation induced by chemical carcinogens and identified a subset of cell transformation-related, concordantly modulated m⁶A sites. Notably, the increased m⁶A in 3'-UTR mRNA of oncogene CDCP1 was found in malignant transformed cells. Mechanistically, the m⁶A methyltransferase METTL3 and demethylases ALKBH5 mediate the m⁶A modification in 3'-UTR of CDCP1 mRNA. METTL3 and m⁶A reader YTHDF1 preferentially recognize m

SUBMITTER: Yang F 

PROVIDER: S-EPMC6756049 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature