Epigenetic reprogramming of primary pancreatic cancer cells counteracts their in vivo tumourigenicity.

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) arises through accumulation of multiple genetic alterations. However, cancer cells also acquire and depend on cancer-specific epigenetic changes. To conclusively demonstrate the crucial relevance of the epigenetic programme for the tumourigenicity of the cancer cells, we used cellular reprogramming technology to reverse these epigenetic changes. We reprogrammed human PDAC cultures using three different techniques - (1) lentivirally via induction of Yamanaka Factors (OSKM), (2) the pluripotency-associated gene OCT4 and the microRNA mir-302, or (3) using episomal vectors as a safer alternative without genomic integration. We found that induction with episomal vectors was the most efficient method to reprogram primary human PDAC cultures as well as prim

SUBMITTER: Khoshchehreh R 

PROVIDER: S-EPMC6756074 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature