Adult Goat Retinal Neuronal Culture: Applications in Modeling Hyperglycemia.

ABSTRACT: Culture of adult neurons of the central nervous system (CNS) can provide a unique model system to explore neurodegenerative diseases. The CNS includes neurons and glia of the brain, spinal cord and retina. Neurons in the retina have the advantage of being the most accessible cells of the CNS, and can serve as a reliable mirror to the brain. Typically, primary cultures utilize fetal rodent neurons, but very rarely adult neurons from larger mammals. Here, we cultured primary retinal neurons isolated from adult goat up to 10 days, and established an in vitro model of hyperglycemia for performing morphological and molecular characterization studies. Immunofluorescence staining revealed that approximately 30-40% of cultured cells expressed neuronal markers. Next, we examined the relative

SUBMITTER: Sharma S 

PROVIDER: S-EPMC6756134 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature