Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachine.

ABSTRACT: Engineering delivery systems for proteins and peptides into mammalian cells is an ongoing challenge for cell biological studies as well as for therapeutic approaches. Photorhabdus luminescens toxin complex (PTC) is a heterotrimeric protein complex able to deliver diverse protein toxins into mammalian cells. We engineered the syringe-like nanomachine for delivery of protein toxins from different species. In addition, we loaded the highly active copepod luciferase Metridia longa M-Luc7 for accurate quantification of injected molecules. We suggest that besides the probable size limitation, the charge of the cargo also influences the efficiency of packing and transport into mammalian cells. Our data show that the PTC constitutes a powerful system to inject recombinant proteins, p

SUBMITTER: Ng Ang A PN 

PROVIDER: S-EPMC6756610 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature