Human γδ T-cell receptor repertoire is shaped by influenza viruses, age and tissue compartmentalisation.

ABSTRACT:

Background

Although γδ T cells comprise up to 10% of human peripheral blood T cells, questions remain regarding their role in disease states and T-cell receptor (TCR) clonal expansions. We dissected anti-viral functions of human γδ T cells towards influenza viruses and defined influenza-reactive γδ TCRs in the context of γδ-TCRs across the human lifespan.

Methods

We performed ⁵¹Cr-killing assay and single-cell time-lapse live video microscopy to define mechanisms underlying γδ T-cell-mediated killing of influenza-infected targets. We assessed cytotoxic profiles of γδ T cells in influenza-infected patients and IFN-γ production towards influenza-infected lung epithelial cells. Using single-cell RT-PCR, we characterised paired TCRγδ clonotypes for influenza-reactive

SUBMITTER: Sant S 

PROVIDER: S-EPMC6756999 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature