Characterization of a sandwich ELISA for quantification of total human soluble neuropilin-1.

ABSTRACT:

Background

Neuropilin-1 (NRP1) is a highly interactive molecule that exists as transmembrane and soluble isoforms. Measurement of circulating levels of soluble NRP1 (sNRP1) in human serum and plasma has proven to be difficult due to present matrix interferences and due to the lack of a reliable technique.

Methods

We developed a highly specific and sensitive sandwich ELISA assay for total sNRP1 quantification in peripheral blood, and we validated the test according to ICH guidelines. The linear epitopes of the employed polyclonal and monoclonal anti-human NRP1 antibodies were mapped with microarray technology. We included a sample pre-treatment step with guanidine hydrochloride (GuHCl) to release sNRP1 from existing interferants.

Results

The ELISA assay which is calibr

SUBMITTER: Gadermaier E 

PROVIDER: S-EPMC6757120 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature