Serine hydroxymethyltransferase controls blood-meal digestion in the midgut of Aedes aegypti mosquitoes.

ABSTRACT:

Background

Female Aedes aegypti mosquitoes are vectors of arboviruses that cause diverse diseases of public health significance. Blood protein digestion by midgut proteases provides anautogenous mosquitoes with the nutrients essential for oocyte maturation and egg production. Midgut-specific miR-1174 affects the functions of the midgut through its target gene serine hydroxymethyltransferase (SHMT). However, less is known about SHMT-regulated processes in blood digestion by mosquitoes.

Methods

RNAi of SHMT was realized by injection of the double-stranded RNA at 16 h post-eclosion. The expression of SHMT at mRNA level and protein level was assayed by real-time PCR and Western blotting, respectively. Statistical analyses were performed with GraphPad7 using Student's t-test.

SUBMITTER: Li X 

PROVIDER: S-EPMC6757384 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature