MiR-205-5p inhibits human endometriosis progression by targeting ANGPT2 in endometrial stromal cells.

ABSTRACT:

Background

miRNA expression profiles in ectopic endometrium (EC) serving as pathophysiologic genetic fingerprints contribute to determining endometriosis progression; however, the underlying molecular mechanisms remain unknown.

Methods

miRNA microarray analysis was used to determine the expression profiling of EC fresh tissues. qRT-PCR was performed to screen miR-205-5p expression in EC tissues. The roles of miR-205-5p and its candidate target gene, angiopoietin-2 (ANGPT2), in endometriosis progression were confirmed on the basis of both in vitro and in vivo systems. miR-205-5p and ANGPT2 expression were measured by in situ hybridization and immunochemistry, and their clinical significance was statistically analysed.

Results

miR-205-5p was screened as a novel suppress

SUBMITTER: Zhou CF 

PROVIDER: S-EPMC6757391 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature