Medulloblastoma rendered susceptible to NK-cell attack by TGFβ neutralization.

ABSTRACT: BACKGROUND:Medulloblastoma (MB), the most common pediatric brain cancer, presents with a poor prognosis in a subset of patients with high risk disease, or at recurrence, where current therapies are ineffective. Cord blood (CB) natural killer (NK) cells may be promising off-the-shelf effector cells for immunotherapy due to their recognition of malignant cells without the need for a known target, ready availability from multiple banks, and their potential to expand exponentially. However, they are currently limited by immune suppressive cytokines secreted in the MB tumor microenvironment including Transforming Growth Factor β (TGF-β). Here, we address this challenge in in vitro models of MB. METHODS:CB-derived NK cells were modified to express a dominant negative TGF-β receptor II (DNRII) us

SUBMITTER: Powell AB 

PROVIDER: S-EPMC6757414 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature