Stability and flexibility of full-length human oligodendrocytic QKI6.

ABSTRACT:

Objective

Oligodendrocytes account for myelination in the central nervous system. During myelin compaction, key proteins are translated in the vicinity of the myelin membrane, requiring targeted mRNA transport. Quaking isoform 6 (QKI6) is a STAR domain-containing RNA transport protein, which binds a conserved motif in the 3'-UTR of certain mRNAs, affecting the translation of myelination-involved proteins. RNA binding has been earlier structurally characterized, but information about full-length QKI6 conformation is lacking. Based on known domains and structure predicitons, we expected full-length QKI6 to be flexible and carry disordered regions. Hence, we carried out biophysical and structural characterization of human QKI6.

Results

We expressed and purified full-length QKI6

SUBMITTER: Raasakka A 

PROVIDER: S-EPMC6757426 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature