Genome-wide RNAi screen in Drosophila reveals Enok as a novel trithorax group regulator.

ABSTRACT: BACKGROUND:Polycomb group (PcG) and trithorax group (trxG) proteins contribute to the specialization of cell types by maintaining differential gene expression patterns. Initially discovered as positive regulators of HOX genes in forward genetic screens, trxG counteracts PcG-mediated repression of cell type-specific genes. Despite decades of extensive analysis, molecular understanding of trxG action and regulation are still punctuated by many unknowns. This study aimed at discovering novel factors that elicit an anti-silencing effect to facilitate trxG-mediated gene activation. RESULTS:We have developed a cell-based reporter system and performed a genome-wide RNAi screen to discover novel factors involved in trxG-mediated gene regulation in Drosophila. We identified more than 200 genes affe

SUBMITTER: Umer Z 

PROVIDER: S-EPMC6757429 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature