Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations.

ABSTRACT:

Background

Sigesbeckia glabrescens Makino (SG) is one of the important plant origins of Sigesbeckiae herba and has been widely used for the treatment of chronic inflammatory diseases in China. However, the underlying anti-inflammatory mechanism of SG is rarely investigated and reported. There are more than 40 kinds of chemical constituents in SG, but the action of the bioactive compounds of SG is still unclear. Therefore, we aimed to systemically investigate the mechanisms behind the anti-inflammatory properties of SG by combining in vitro and in silico investigations.

Methods

Cytotoxicity was measured using the 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Nitric oxide (NO) release was detected using the Gr

SUBMITTER: Zhong Z 

PROVIDER: S-EPMC6757439 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature