Identification and characterization of the feeding circuit-activating peptides, a novel neuropeptide family of aplysia.

ABSTRACT: We use a multidisciplinary approach to identify, map, and characterize the bioactivity of modulatory neuropeptides in the circuitry that generates feeding behavior in Aplysia. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of the cerebral-buccal connective (CBC), a nerve containing axons of many interneurons that control feeding behavior of Aplysia, was used to identify neuropeptides that may participate in generation and shaping of feeding motor programs. Using this functionally oriented search, we identified a novel family of peptides that we call the feeding circuit-activating peptides (FCAPs). Two peptides with masses identical to those observed in the CBCs (molecular weight 1387 and 1433) were purified from buccal ganglia and partially sequenced using mas

SUBMITTER: Sweedler JV 

PROVIDER: S-EPMC6757975 | biostudies-literature | 2002 Sep

REPOSITORIES: biostudies-literature