High-Throughput Single-Cell Transcriptome Profiling of Plant Cell Types.

ABSTRACT: Single-cell transcriptome profiling of heterogeneous tissues can provide high-resolution windows into developmental dynamics and environmental responses, but its application to plants has been limited. Here, we used the high-throughput Drop-seq approach to profile >12,000 cells from Arabidopsis roots. This identified numerous distinct cell types, covering all major root tissues and developmental stages, and illuminated specific marker genes for these populations. In addition, we demonstrate the utility of this approach to study the impact of environmental conditions on developmental processes. Analysis of roots grown with or without sucrose supplementation uncovers changes in the relative frequencies of cell types in response to sucrose. Finally, we characterize the transcriptome changes t

SUBMITTER: Shulse CN 

PROVIDER: S-EPMC6758921 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature