A Recombinant Attenuated Yersinia pseudotuberculosis Vaccine Delivering a Y. pestis YopE_Nt138-LcrV Fusion Elicits Broad Protection against Plague and Yersiniosis in Mice.

ABSTRACT: In this study, a novel recombinant attenuated Yersinia pseudotuberculosis PB1+ strain (χ10069) engineered with ΔyopK ΔyopJ Δasd triple mutations was used to deliver a Y. pestis fusion protein, YopE amino acid 1 to 138-LcrV (YopE_Nt138-LcrV), to Swiss Webster mice as a protective antigen against infections by yersiniae. χ10069 bacteria harboring the pYA5199 plasmid constitutively synthesized the YopE_Nt138-LcrV fusion protein and secreted it via the type 3 secretion system (T3SS) at 37°C under calcium-deprived conditions. The attenuated strain χ10069(pYA5199) was manifested by the establishment of controlled infection in different tissues without developing conspicuous signs of disease in histopathological analysis of microtome sections

SUBMITTER: Singh AK 

PROVIDER: S-EPMC6759313 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature