Effect of Iron Oxide Nanoparticles on the Oxidation and Secondary Structure of Growth Hormone.

ABSTRACT: Oxidation of therapeutic proteins (TPs) can lead to changes in their pharmacokinetics, biological activity and immunogenicity. Metal impurities such as iron are known to increase oxidation of TPs, but nanoparticulate metals have unique physical and chemical properties compared to the bulk material or free metal ions. Iron oxide nanoparticles (IONPs) may originate from equipment used in the manufacturing of TPs or from needles during injection. In this study, the impact of IONPs on oxidation of a model protein, rat growth hormone (rGH), was investigated under chemical stress. Hydrogen peroxide (H₂O₂)- and 2,2'-azobis (2-methylpropionamidine) dihydrochloride oxidized methionine residues of rGH, but unexpectedly, oxidation was suppressed in the presence of IONPs compared

SUBMITTER: Varkhede N 

PROVIDER: S-EPMC6759409 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature