Transient Focal Ischemia Significantly Alters the m⁶A Epitranscriptomic Tagging of RNAs in the Brain.

ABSTRACT: Background and Purpose- Adenosine in many types of RNAs can be converted to m⁶A (N⁶-methyladenosine) which is a highly dynamic epitranscriptomic modification that regulates RNA metabolism and function. Of all organs, the brain shows the highest abundance of m⁶A methylation of RNAs. As recent studies showed that m⁶A modification promotes cell survival after adverse conditions, we currently evaluated the effect of stroke on cerebral m⁶A methylation in mRNAs and lncRNAs. Methods- Adult C57BL/6J mice were subjected to transient middle cerebral artery occlusion. In the peri-infarct cortex, m⁶A levels were measured by dot blot analysis, and transcriptome-wide m⁶A changes were profiled using immunoprecipitated methylated

SUBMITTER: Chokkalla AK 

PROVIDER: S-EPMC6759411 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature