Histopathological factors affecting the extraction of high quality genomic DNA from tissue sections for next-generation sequencing.

ABSTRACT: To enable the widespread application of genomic medicine, the extraction of genomic DNA from thin sections of archived formalin-fixed and paraffin-embedded (FFPE) tissue blocks for next-generation sequencing (NGS) is often necessary. However, there are currently no guidelines available on which specific regions of the microtome sections to use for macrodissection with respect to the histopathological factors observed under microscopic examination. The aim of this study was to clarify the relationship between histopathological factors and DNA quality, and to standardize the macrodissection method for more efficient implementation of NGS. FFPE tissue specimens of 218 patients from the Biomarker Research for Anti-EGFR Monoclonal Antibodies by Comprehensive Cancer Genomics study were used to i

SUBMITTER: Fujii S 

PROVIDER: S-EPMC6759515 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature