Genomic landscape of epithelium with low-grade atypia on gastric cancer after Helicobacter pylori eradiation therapy.

ABSTRACT:

Background

Gastric cancer may develop after successful eradication of Helicobacter pylori, although the incidence is lower than in non-eradicated individuals. We previously reported the appearance of characteristic epithelium with low-grade atypia (ELA) on the surface of gastric cancer after H. pylori eradication. However, whether ELA originates from cancer after re-differentiation or from the non-cancerous surrounding mucosa is unknown.

Methods

We isolated ELA regions from 10 early gastric cancer patients and analyzed the nucleotide sequences for 90 oncogenes and 35 fusion oncogenes, comparing them with counterpart cancer tissue, normal gastric mucosa, and blood cell-derived DNA. Somatic mutations in each tissue were identified by comparing them with the sequences from whol

SUBMITTER: Masuda K 

PROVIDER: S-EPMC6759680 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature