Radioresistance of Serpinb3a-/- Mice and Derived Hematopoietic and Marrow Stromal Cell Lines.

ABSTRACT: Serpins are a group of serine-proteases involved in multiple signal transduction pathways in mammalian cells. In particular, Serpinb3a is involved in the lysosomal necrosis cell death pathway with components that overlap with radiation-induced apoptosis. We investigated the radiation response of Serpinb3a^-/- mice compared to Serpinb3a^+/+ mice on the Balb/c background. Serpinb3a^-/- mice showed significant radioresistance to a dose of 8.0 Gy total-body irradiation, compared to Serpinb3a^+/+ Balb/c mice. Long-term bone marrow cultures from Serpinb3a^-/- mice showed increased longevity. In clonogenic survival assays, fresh bone marrow hematopoietic progenitors, as well as clonal interleukin-3 (IL-3)-dependent hematopoietic progenitor and bon

SUBMITTER: Thermozier S 

PROVIDER: S-EPMC6759811 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature