Examining the Protein Interactome and Subcellular Localization of RNase J2 Complexes in Streptococcus mutans.

ABSTRACT: Regulated RNA turnover is vital for the control of gene expression in all cellular life. In Escherichia coli, this process is largely controlled by a stable degradosome complex containing RNase E and a variety of additional enzymes. In the Firmicutes phylum, species lack RNase E and often encode the paralogous enzymes RNase J1 and RNase J2. Unlike RNase J1, surprisingly little is known about the regulatory function and protein interactions of RNase J2, despite being a central pleiotropic regulator for the streptococci and other closely related organisms. Using crosslink coimmunoprecipitation in Streptococcus mutans, we have identified the major proteins found within RNase J2 protein complexes located in the cytoplasm and at the cell membrane. In both subcellular fractions, RN

SUBMITTER: Mu R 

PROVIDER: S-EPMC6759994 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature