Use of GelMA for 3D printing of cardiac myocytes and fibroblasts.

ABSTRACT:

Aim

To 3D print heart tissue, one must understand how the main two types of cardiac cells are affected by the printing process.

Materials & methods

Effects of gelatin methacryloyl (GelMA) concentration, extruder pressure and duration of UV exposure on survival of cardiac myocytes and fibroblasts were examined using lactate dehydrogenase and LIVE/DEAD assays, bioluminescence imaging and morphological assessment.

Results & conclusion

Cell survival within 3D printed cardiomyocyte-laden GelMA constructs was more sensitive to extruder pressure and GelMA concentrations than within 3D fibroblast-laden GelMA constructs. Cells within both types of constructs were adversely impacted by the UV curing step. Use of mixed cell populations and enrichment of bioink formulation with f

SUBMITTER: Koti P 

PROVIDER: S-EPMC6760315 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature