Automated serial rotation electron diffraction combined with cluster analysis: an efficient multi-crystal workflow for structure determination.

ABSTRACT: Serial rotation electron diffraction (SerialRED) has been developed as a fully automated technique for three-dimensional electron diffraction data collection that can run autonomously without human intervention. It builds on the previously established serial electron diffraction technique, in which submicrometre-sized crystals are detected using image processing algorithms. Continuous rotation electron diffraction (cRED) data are collected on each crystal while dynamically tracking the movement of the crystal during rotation using defocused diffraction patterns and applying a set of deflector changes. A typical data collection screens up to 500 crystals per hour, and cRED data are collected from suitable crystals. A data processing pipeline is developed to process the SerialRED data sets.

SUBMITTER: Wang B 

PROVIDER: S-EPMC6760450 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature