Molecular characterization of MHC II DRB3 gene of swamp- and riverine-type water buffaloes.

ABSTRACT:

Objective

Major histocompatibility complex (MHC) is a set of molecular proteins on the surface of antigen presenting cells encoded by a large gene family which are important parts of the immune system. This study was conducted to convey information on the genetic characteristics of the MHC II DRB3 gene in riverine and swamp buffaloes.

Materials and methods

Characterization of MHC II DRB3 gene was carried out using polymerase chain reaction (PCR)-based assay. Thirty-milliliter milk samples were collected from 10 swamp-type and 10 riverine-type buffaloes. RNA from milk samples were extracted using Trizol and then followed by reverse transcription-PCR (RT-PCR).

Results

The phylogenetic analysis with 1,000 bootstrap replications clearly showed complex parsimony in MHC II

SUBMITTER: Medina NP 

PROVIDER: S-EPMC6760514 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature