Fluorescent blood-brain barrier tracing shows intact leptin transport in obese mice.

ABSTRACT:

Background/objectives

Individuals carrying loss-of-function gene mutations for the adipocyte hormone leptin are morbidly obese, but respond favorably to replacement therapy. Recombinant leptin is however largely ineffective for the vast majority of obese individuals due to leptin resistance. One theory underlying leptin resistance is impaired leptin transport across the blood-brain-barrier (BBB). Here, we aim to gain new insights into the mechanisms of leptin BBB transport, and its role in leptin resistance.

Methods

We developed a novel tool for visualizing leptin transport using infrared fluorescently labeled leptin, combined with tissue clearing and light-sheet fluorescence microscopy. We corroborated these data using western blotting.

Results

Using 3D whole brain i

SUBMITTER: Harrison L 

PROVIDER: S-EPMC6760579 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature