A novel strategy for interpreting the T-SPOT.TB test results read by an ELISPOT plate imager.

ABSTRACT: BACKGROUND:The T-SPOT.TB can be read by an ELISPOT plate imager as an alternative to a labor-intensive and time-consuming manual reading, but its accuracy has not been sufficiently discussed to date. METHODS:1,423 test results obtained from manual reading using a microscope and an ELISPOT plate imager were compared. The agreement of qualitative test results was assessed using Cohen's kappa coefficient. The relationship of spot counts was studied using Bland-Altman analysis. RESULTS:The overall percent agreement of the qualitative test results was 95.43% with a kappa coefficient of 0.91. Positive test results with the maximum net spot count of 8 and borderline test results showed relatively high discordance. The agreement of spot counts in panel A, panel B, and nil control was good, and var

SUBMITTER: Kim TY 

PROVIDER: S-EPMC6760805 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature